Citation Information :
Shri R, Kaushik S, Singh V, Anand A. Electrophysiological assessment of retinal functions by ERG in Ischemia/Reperfusion (I/R) Allium cepa pre-treated mice. Integr Med Case Rep 2020; 1 (1):18-28.
Retinal disorders are the one of the most challenging and complex degenerative diseases that need to be addressed because of rapid increase in the number of affected individuals. Most of the available treatments strategies are inadequate to exert permanent solution to the patients. Therefore, as an alternative approach we wanted to test the efficacy of Allium cepa (A. cepa) in an Ischemia/Reperfusion (I/R) mouse model. We orally administered the aqueous extract of A. cepa at different dosages 100 mg/kg, 200 mg/kg, 300 mg/kg 24 hrs prior to the surgery. Electroretinogram (ERG) analysis was carried out at 7 day, 21 day, and 28 day after the surgery. ERG recording depicted that A. cepa administration is able to increase the implicit time but not at the statistically significant level for which larger sample size and deeper analysis is required.
Essa MM, Akbar M, Guillemin G. The Benefits of Natural Products for Neurodegenerative Diseases: Springer; 2016.
Sofi F, Dinu MR. Nutrition and prevention of chronic-degenerative diseases. Agriculture and agricultural science procedia. 2016;8:713–7.
Jiang X, Huang J, Song D, Deng R, Wei J, Zhang Z. Increased Consumption of Fruit and Vegetables Is Related to a Reduced Risk of Cognitive Impairment and Dementia: Meta-Analysis. Frontiers in aging neuroscience. 2017;9:18.
Sarchielli E, Morelli A, Guarnieri G, Iorizzi M, Sgambati E. Neuroprotective effects of quercetin 4'-O-β-d-diglucoside on human striatal precursor cells in nutrient deprivation condition. Acta histochemica. 2018;120(2):122–8.
Shri R, Bora KS. Neuroprotective effect of methanolic extracts of Allium cepa on ischemia and reperfusion-induced cerebral injury. Fitoterapia. 2008;79(2):86–96.
Nejabat M, Salehi A, Noorani Azad P, Ashraf MJ. Effects of onion juice on the normal flora of eyelids and conjunctiva in an animal model. Jundishapur journal of microbiology. 2014;7(5):e9678–e.
Javadzadeh A, Ghorbanihaghjo A, Bonyadi S, Rashidi MR, Mesgari M, Rashtchizadeh N, et al. Preventive effect of onion juice on selenite-induced experimental cataract. Indian journal of ophthalmology. 2009;57(3):185.
Stefek M, Karasu C. Eye lens in aging and diabetes: effect of quercetin. Rejuvenation research. 2011;14(5):525–34.
Agte V, Tarwadi K. The importance of nutrition in the prevention of ocular disease with special reference to cataract. Ophthalmic research. 2010;44(3):166–72.
Sato A, Zhang T, Yonekura L, Tamura H. Antiallergic activities of eleven onions (Allium cepa) were attributed to quercetin 4'-glucoside using QuEChERS method and Pearson's correlation coefficient. Journal of Functional Foods. 2015;14:581–9.
Singh T, Goel RK. Neuroprotective effect of Allium cepa L. in aluminium chloride induced neurotoxicity. Neurotoxicology. 2015;49:1–7.
Movahedian A, Sadeghi H, Ghannadi A, Gharavi M, Azarpajooh S. Hypolipidemic activity of Allium porrum L. in cholesterol-fed rabbits. Journal of medicinal food. 2006;9(1):98–101.
Kumari K, Augusti K. Lipid lowering effect of S-methyl cysteine sulfoxide from Allium cepa Linn in high cholesterol diet fed rats. Journal of ethnopharmacology. 2007;109(3):367–71.
Tamtaji OR, Hosseinzadeh H, Talaei SA, Behnam M, Firoozeh SMT, Taghizadeh M, et al. Protective Effects of Red Onion (Allium cepa) Ethanolic Extract on Learning and Memory Impairments in Animal Models of Diabetes. Galen Medical Journal. 2017;6(3):249–57.
Vauzour D, Vafeiadou K, Rodriguez-Mateos A, Rendeiro C, Spencer JPE. The neuroprotective potential of flavonoids: a multiplicity of effects. Genes & nutrition. 2008;3(3-4):115–26.
Minhas G, Morishita R, Anand A. Preclinical models to investigate retinal ischemia: advances and drawbacks. Frontiers in neurology. 2012;3:75.
Tomiyama Y, Fujita K, Nishiguchi KM, Tokashiki N, Daigaku R, Tabata K, et al. Measurement of electroretinograms and visually evoked potentials in awake moving mice. PloS one. 2016;11(6).
Hema L. Ramkumar KDE, Koushik Tripathy, Peter A. Karth, Usha Rajapuram Kumar, and Vinay A. Shah. Electroretinogram: EyeWiki; [updated 15 November 2019. Available from: https://eyewiki.aao.org/Electroretinogram.
Neuroscience Research Lab DoN, Post Graduate Institute of Medical Education and Research, Chandigarh. Redefining Quality Standards in Basic Research Investigations by Broadening the Purview of GLP: Quality Council of India.
Bammidi S, Sharma K, Tyagi R, Sharma NK, Anand A. Eye Genetics: The Road Ahead to Quality Standards. Advances in Vision Research, Volume I: Springer; 2017. p. 473–80.
Modgil S, Cameotra SS, Sharma VL, Anand A. Early Life Pb Exposure and its Effect on Later Life Retinal Degeneration. Journal of cellular biochemistry. 2017.
Minhas G, Prabhakar S, Morishita R, Shimamura M, Bansal R, Anand A. Transplantation of lineage-negative stem cells in pterygopalatine artery ligation induced retinal ischemia–reperfusion injury in mice. Molecular and cellular biochemistry. 2017;429(1–2):123–36.
Kumar S, Modgil S, Bammidi S, Minhas G, Shri R, Kaushik S, et al. Allium cepa exerts neuroprotective effect on retinal ganglion cells of pterygopalatine artery (PPA) ligated mice. Journal of Ayurveda and Integrative Medicine. 2020.
Farkouh A, Frigo P, Czejka M. Systemic side effects of eye drops: a pharmacokinetic perspective. Clinical Ophthalmology (Auckland, NZ). 2016;10:2433.
Brandli A, Stone J. Using the electroretinogram to assess function in the rodent retina and the protective effects of remote limb ischemic preconditioning. JoVE (Journal of Visualized Experiments). 2015(100):e52658.
Hancock HA, Kraft TW. Oscillatory potential analysis and ERGs of normal and diabetic rats. Investigative ophthalmology & visual science. 2004;45(3):1002-8.
